While they chose to reject the five substitute proposal, the Premier League clubs have voted in principle to introduce permanent concussion substitutions.
Teams will be able to use up to two additional replacements per game if players are suspecting of sustaining, or diagnosed with, a concussion.
The move comes after the International Football Association Board (IFAB) - which governs the laws of the game - approved the rule change on a trial basis on Wednesday.
The Premier League will now apply to IFAB to take part in the trial, which begins next month.
The FA have said they will allow 5 subs in FA Cup matches.